Guardian.co.uk held first position once again with an increase of 4.65 per cent, with unique users now at 24,186,422.
The monthly audit measures the number of unique users/browsers to each registered news site.
In close second was Telegraph.co.uk with a rise of 885,986 to 22,945,934 unique users, an increase of 4.02 per cent.
In third place was Times Online, up by 631,875 to 20,322,634 unique users - an increase of 3.21 per cent.
Independent.co.uk recorded the largest increase for an online broadsheet though it still remains lowest of the four titles, at 7,995,958 unique users. This figure was up from 6,629,235 unique users last month - a 20.62 per cent increase.
This month's ABCe figures were a 'record high' for Telegraph.co.uk, a release from the group said.
"Telegraph.co.uk benefited from a surge in demand for stories about the US election and the financial crisis, which helped raise the number of page views and unique users to a record high," said Edward Roussel, digital editor at the Telegraph Media Group, in the release.
"We continued to innovate in September with two key projects: Corduroy Mansions, the online novel written by Alexander McCall Smith, and Britain at War, a project that pulls together our readers' recollections of the Second World War," he said.
Of the main tabloid news sites only Mail Online saw an increase. It went up by 445,616 from last month to 17,913,660 unique users, a 2.55 per cent increase.
Sun Online went down by 201,741 to 15,783,551 unique users for September - a decrease of 1.26 per cent.
The Mirror site also dropped by 315,245 to 5,259,763 unique users, down 5.65 per cent, though, according to a release issued today, the site recorded a 60 per cent rise in unique users year-on-year.
"The development of mirror.co.uk is ongoing and we continue to grow an engaged, UK audience delivering strong year-on-year growth," said Mark Hollinshead, managing director, Trinity Mirror Nationals division.
Despite the monthly drop, the release from the Mirror Group emphasised a growth in UK audience since last year (up 90 per cent), and that 58.4 per cent of the audience are from within the UK, compared with 41.6 per cent who are overseas users.
Significant year-on-year increases were seen in unique users across the board: 53.24 per cent for Mail Online; 47.68 per cent for the Sun; 62.11 per cent for Times Online; and 44.78 per cent for Guardian.co.uk.
The Independent's site gained 96.83 per cent in unique users since September 2007. The Telegraph, however, led the way - with a year-on-year jump in unique users of 115.55 per cent.
The Express' site and FT.com do not record their monthly audit figures with ABCe.
In the last report, which recorded the figures for August, the Guardian.co.uk, Telegraph.co.uk and Times Online recorded substantial gains in unique users, with the Guardian coming out top.
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