Staff cameraman Ali Hassan Al Jaber, a Qatari national, was reportedly shot on Saturday when the vehicle he and other Al Jazeera staff were travelling in was attacked.
He was was taken to hospital but died from his wounds. A colleague who was also in the vehicle, Nasser Al-Haddar, a Libyan national, was wounded in the attack and is in hospital.
In a statement on its website, the broadcaster accused forces loyal to Gaddafi of a "malicious campaign targeting Al Jazeera and its staff".
"Al Jazeera reiterates the assault cannot dent its resolve to continue its mission, professionally enlightening the public of the unfolding events in Libya and elsewhere.
"Al Jazeera stresses it will relentlessly prosecute and bring to justice all perpetrators and their accomplices."
Reporters without Borders has said the organisation is "outraged" by the killing.Al Jaber's death follows reports last week that a BBC Arabic crew were captured, tortured and subjected to a mock execution.
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